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What are the two ingredients in lysis solution?

Amelia Kim | 2023-05-28 16:03:29 | page views：1965
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Zoe Brown

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RIPA (RadioImmunoPrecipitation Assay) lysis buffer1% (w/w) Nonidet P-40 (NP-40)1% (w/v) sodium deoxycholate.0.1% (w/v) SDS.0.15 M NaCl.0.01 M sodium phosphate, pH 7.2.2 mM EDTA.50 mM sodium fluoride (NaF)0.2 mM fresh sodium orthovanadate (Na3VO4.2H2O, it has phosphatase inhibitor function because it mimics phosphate)More items...
2023-05-28 16:03:29
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Ethan Brown

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RIPA (RadioImmunoPrecipitation Assay) lysis buffer1% (w/w) Nonidet P-40 (NP-40)1% (w/v) sodium deoxycholate.0.1% (w/v) SDS.0.15 M NaCl.0.01 M sodium phosphate, pH 7.2.2 mM EDTA.50 mM sodium fluoride (NaF)0.2 mM fresh sodium orthovanadate (Na3VO4.2H2O, it has phosphatase inhibitor function because it mimics phosphate)More items...
Lysis buffer - Wikipedia
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