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What does chloroform do in RNA isolation?

Ethan Gonzales | 2023-05-28 11:29:35 | page views：1550
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Harper Collins

Studied at the University of Barcelona, Lives in Barcelona, Spain.
Purification of RNA using TRIzol (TRI reagent). ... After solubilization, the addition of chloroform causes phase separation (much like extraction with phenol:chloroform:isoamyl alcohol), where protein is extracted to the organic phase, DNA resolves at the interface, and RNA remains in the aqueous phase.
2023-06-05 11:29:35
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Benjamin Patel

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Purification of RNA using TRIzol (TRI reagent). ... After solubilization, the addition of chloroform causes phase separation (much like extraction with phenol:chloroform:isoamyl alcohol), where protein is extracted to the organic phase, DNA resolves at the interface, and RNA remains in the aqueous phase.
Purification of RNA using TRIzol (TRI reagent). - NCBI
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